Tutorials#

A gallery of examples that showcase how the PhasorPy library can be used to analyze luminescence lifetime and hyperspectral images using the Phasor approach.

Some tutorials demonstrate the use of the programming interface in general, while others provide problem-oriented how-to guides for advanced applications.

Introduction to PhasorPy

Introduction to PhasorPy

Geometric interpretation of lifetimes

Geometric interpretation of lifetimes

LFD Workshop FLIM tutorial

LFD Workshop FLIM tutorial

Applications#

Problem-oriented how-to guides for advanced applications:

Spectral unmixing

Spectral unmixing

FRET efficiency image

FRET efficiency image

Bound NADH fraction

Bound NADH fraction

Absolute NADH concentration

Absolute NADH concentration

Multidimensional phasor approach

Multidimensional phasor approach

Programming interface#

Tutorials on specific modules, classes, and functions:

File input/output

File input/output

Signal synthesis from lifetimes

Signal synthesis from lifetimes

Phasor coordinates from lifetimes

Phasor coordinates from lifetimes

Förster resonance energy transfer

Förster resonance energy transfer

Filter phasor coordinates

Filter phasor coordinates

Multi-harmonic phasor coordinates

Multi-harmonic phasor coordinates

Phasor plot

Phasor plot

Cursors

Cursors

Multi-component analysis

Multi-component analysis

Principal component analysis

Principal component analysis

Miscellaneous#

Tutorials that do not fit into other categories:

PhasorPy logo

PhasorPy logo

Interactive applications

Interactive applications

Benchmark phasor_from_signal

Benchmark phasor_from_signal

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